Donnish Journal of Medicine and Medical Sciences

April 2015 Vol. 2(3), pp. 032-035

Copyright © 2015 Donnish Journals

Original Research Paper

The Role of Interferon-gamma (IFN-γ) and Interleukin-10 (IL-10) in Iraqi Patients with Visceral Leishmaniasis (VL)

¹Jabbar R. Zangor Al-Autabbi, ²Abdulsadah A. Rahi* and ³Ali Khamesipour

¹Department Microbiology, College of Medicine, Baghdad University, Baghdad, Iraq
²Department of Biology, College of Science, Wasit University, Kut, Iraq
³Center of Research and Training in Skin Diseases and Leprosy, TUMS, Tehran, Iran

Corresponding Author E-mail: abdulsadah1966@yahoo.com

Accepted 4th April, 2015.

Abstract

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A total of 50 blood samples of active visceral leishmaniasis was identified clinically by fever of more than two-week duration, hepatosplenomegaly, and pancytopenia then confirmed by rK39 Dipstick. Fifty bone marrow smears showed moderate to sever megaloblastosis, an increased number of plasma cells and megakaryocytic hyperplasia with abnormal morphology. Amastigotes appeared as round or oval bodies found intracellular in monocytes and macrophages in the stained smears. Peripheral blood samples and lymph nodes were obtained before and 30 days after, completion of treatment with sodium stibogluconate (Pentostam;UK). Enzyme immunoassay was carried for quantitative determination of human IFN-γ and IL-10 in serum of patient and control groups. Serum IFN-gamma level showed the distribution of serum levels of IFN-gamma in patients with VL and control group. The levels in the pretreated VL group were (60.87± 3.6) pg/ml higher than control (5.12± 0.98) pg/ml with a significant difference (p<0.01). There was a significant decrease in serum levels of IFN-gamma in post treated group (9.83± 1.1) pg/ml, which were significantly lower than that in the pretreated group. There was no significant difference between post treated and control group (p>0.05). Serum levels of IL-10 in the control were (2.98± 0.52)pg/ml significantly lower than that in patients with VL(110.8 ±4.2) pg/ml. In the post-treated VL group the levels were (4.75±0.86) pg/ml which were significantly lower than pretreated VL group (p<0.01). There was no significant difference between post treated and control group (p>0.05).

Keywords: VL, Human, IL-10, IFN-γ.

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Cite This Article:

Jabbar R. Zangor Al-Autabbi, Abdulsadah A. Rahi and Ali Khamesipour. The Role of Interferon-gamma (IFN-γ) and Interleukin-10 (IL-10) in Iraqi Patients with Visceral Leishmaniasis (VL). Donnish Journal of Medicine and Medical Sciences 2(3) 2015 pp. 032-035.